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. 2007 Nov 5;179(3):411–421. doi: 10.1083/jcb.200706176

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Cell cycle regulation of γ satellite transcription in WT and Suv39h1,2 double knockout MEFs. Asynchronously growing WT and Suv39h1,2 double null cell line (D15) MEFs were subjected to PCNA and γ satellite RNA-FISH analysis as in Fig. 3. (A) The percentage of cells with RNA-FISH signals and the range of signals per cell were scored and plotted as in Fig. 3. (B) The percentage of cells with at least one (WT) or seven (D15) sites of γ satellite transcription revealed as RNA-FISH foci were scored as in Fig. 3. (C) RNA-FISH foci were scored in prophase/metaphase (P/M) and anaphase/telophase (A/T) cells as in Fig. 2 B.