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. 2007 Nov 5;179(3):485–500. doi: 10.1083/jcb.200702115

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Ubiquitin and p62 positive structures accumulate in cells expressing CHMP2 mutants. HeLa cells transfected with myc-tagged wild-type CHMP2B (A), CHMP2B^Intron5 (B), or CHMP2B^Δ10 (C) were labeled with antibodies against c-Myc (red), ubiquitin (green), and p62 (blue) and analyzed by confocal microscopy. Single channel images in black and white are shown. Bars,10 μm. (D) Total levels of p62 in these cells were quantified in 20 cells from three independent experiments using the Zeiss LSM 510 Meta software and the average normalized to the average p62 level in control (mock transfected) cells. Error bars = SEM. (E) Western blot analysis showing accumulation of p62 in cells transfected with myc-CHMP2B^Intron5 compared with mock-transfected cells (control) and cells transfected with CHMP2B^wt. (F) Immuno-EM showing p62-positive aggregates containing dense clusters of vesicular–tubular structures in cells transfected with myc-CHMP2B^Intron5. Bar, 200 nm.