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. 2003 Oct 1;31(19):e115. doi: 10.1093/nar/gng113

Figure 3.

Figure 3

Modifications to the tagged microarray approach. (a) Using tag detectors labelled with different fluorochromes produces different fluorescent outputs from the same samples. Sample JI 2698 contains an occupied 1794-1 allele and sample Therèse is unoccupied. Samples were spotted in triplicate. (b) Using four tag detectors to analyse the allelic state for two loci in the same reaction. Sample JI 2698 is occupied for 1794-1 and unoccupied for UniTpv, sample Therèse is unoccupied for 1794-1 and occupied for UniTpv and sample JI 2055 is unoccupied for both loci. Tag detectors used for both loci produce Cy3 signal (green) from an occupied allele and Cy5 signal (red) from an unoccupied allele.