Table 1. Oligonucleotides used in this study.
Name | Type | Sequence |
---|---|---|
Biotin-1794L | Common biotinylated primer | [biotin]CTTCATGTGAATGAACGCAC |
A-C18-1794-1R | Unoccupied site Tag primer | TCTTTGAGTTTGACCATGCAACGTGAGCGA[18]AAGAACATTGAAGCTTTATCCTTG |
C-SP-PBS(–) | Occupied site Tag primer | GCCATACAATAGTCACGTTGGAGTTGGACA[18]CGGGAGTTCTTGATACCATGTT |
Cy5-A-B | Tag detector oligonucleotide | [Cy5]TCTTTGAGTTTGACCATGCAACGTGAGCGACAATCAGGACGGCTACGTGCAATACTTAGT |
Cy5-A′-B′ | Tag detector oligonucleotide | [Cy5]TCGCTCACGTTGCATGGTCAAACTCAAAGAACTAAGTATTGCACGTAGCCGTCCTGATTG |
Cy3-C-D | Tag detector oligonucleotide | [Cy3]GCCATACAATAGTCACGTTGGAGTTGGACACCTACTGAATACACTTATACCGCTTACGAG |
Cy3-C′-D′ | Tag detector oligonucleotide | [Cy3]TGTCCAACTCCAACGTGACTATTGTATGGCCTCGTAAGCGGTATAAGTGTATTCAGTAGG |
Biotin-206L | Common SNP206 biotinylated primer | [biotin]TGTCATACCACCCAACTATTCCCCCTACG |
Biotin-957R | Common SNP957 biotinylated primer | [biotin]AAATTCGGCAGCTGGAGCTTGAAATGGAG |
A-SP-206C | SNP206 C allele tag primer | TCTTTGAGTTTGACCATGCAACGTGAGCGA[18]GCCGTACTTCCATTAGCTCTTCGCCCC |
C-SP-206T | SNP206 T allele tag primer | GCCATACAATAGTCACGTTGGAGTTGGACA[18]CGCCGTACTTCCATTAGCTCTTCGAACT |
E-SP-957A | SNP957 A allele tag primer | ACCGCATCCGAACATTTGTCAGTTGAGCAT[18]TGATAACCCCTGATTGAATATGGGCACA |
G-SP-957G | SNP957 G allele tag primer | GCCGATAATCACCTTGTCACTGCTTGAACA[18]TGATAACCCCTGATTGAATATGGGCCAG |
Cy5-E-F | Tag detector oligonucleotide | [Cy5]ACCGCATCCGAACATTTGTCAGTTGAGCATTCTGCCTAAGCCCACTATTCCATCAAGTCT |
Cy5-E′-F′ | Tag detector oligonucleotide | [Cy5]ATGCTCAACTGACAAATGTTCGGATGCGGTAGACTTGATGGAATAGTGGGCTTAGGCAGA |
Cy3-G-H | Tag detector oligonucleotide | [Cy3]GCCGATAATCACCTTGTCACTGCTTGAACAACTCCGAAGTTGCCAATCTTGCGTCAATAG |
Cy3-G′-H′ | Tag detector oligonucleotide | [Cy3]TGTTCAAGCAGTGACAAGGTGATTATCGGCCTATTGACGCAAGATTGGCAACTTCGGAGT |
[18], C-18 spacer.