Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 May 7;177(3):465–476. doi: 10.1083/jcb.200610005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Dp-actin photoactivated in the cytoplasm is efficiently incorporated into the lamellipodium, and the rate of incorporation is dependent on the G-actin pool in the cytoplasm. (A–C) Dp-actin photoactivated in the cytoplasm is incorporated into the lamellipodium. NIE-115 cells were cotransfected with Dp-actin and RacV12 (A); Dp-actin, RacV12, and LIMK1 (B); or Dp-actin, RacV12, LIMK1, and cofilin(S3A) (C). After cell-wide photobleaching, a 14.25- × 2.85-μm rectangular region (white box) was photoactivated, and fluorescence images were acquired every 2 s for 20 s at 37°C using a laser-scanning confocal imaging system to measure the fluorescence decay of Dp-actin (shown in D). The same cells were photobleached, and the same rectangular region was again photoactivated. Fluorescence images were acquired every 5 s for 40 s to measure the incorporation of Dp-actin into the tip of the lamellipodium (A–C; see Video 5, available at http://www.jcb.org/cgi/content/full/jcb.200610005/DC1). Bar, 20 μm. (D) Time course of the fluorescence decay of Dp-actin in the photoactivated region. Plasmids transfected into the cells are indicated on the right. (E) The rate of Dp-actin incorporation into the lamellipodium, measured as the distance of Dp-actin fluorescence advanced from the tip of the lamellipodium toward the cytoplasm for 20 or 40 s after photoactivation. Data in D and E are means ± SEM of 36 (Dp-actin), 20 (Dp-actin; LIMK1[WT]), 33 (Dp-actin; LIMK1[WT]; cofilin[S3A]), 26 (Dp-actin; LIMK1[WT]; cofilin[WT]), and 23 cells (Dp-actin; LIMK1[WT]; cofilin[S3E]). *, P < 0.05, compared with cells expressing LIMK1(WT) alone. (F) The correlation between the fluorescence decay of Dp-actin at 2 s after photoactivation and the rate of Dp-actin incorporation into the lamellipodium. Each point represents an individual cell transfected with the plasmids indicated in D.