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. 2007 May 7;177(3):465–476. doi: 10.1083/jcb.200610005

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Dp-actin photoactivated in the front region of the lamellipodium flows retrogradely and diffuses into the cytoplasm. (A) Fluorescence images of Dp-actin photoactivated in the front region of the lamellipodium in NIE-115 cells expressing Dp-actin and RacV12. After cell-wide photobleaching, a 14.25- × 2.85-μm rectangular region (white box) was photoactivated, and fluorescence images were acquired every 5 s for 90 s at 37°C using a laser-scanning confocal imaging system. See Video 6 (available at http://www.jcb.org/cgi/content/full/jcb.200610005/DC1). Bar, 10 μm. (B) Time-dependent changes in fluorescence intensity of Dp-actin in the front and the rear regions of the lamellipodium (left) with correction for photobleaching. The mean fluorescence intensity in the front region immediately after photoactivation (t = 0 s) was set to 100%. Data are means ± SEM of 25 cells from three independent experiments.