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. 2007 May 7;177(3):425–437. doi: 10.1083/jcb.200611166

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Emi1 is required to accumulate cyclins A and B1. (A) Cumulative number of cells entering mitosis (NEBD) plotted over time, as observed by time-lapse DIC microscopy of cells transfected with either control or Emi1_1 siRNA. Representative of three experiments. (B) Emi1 and cyclins A and B1 levels in cells transfected with either control or siEmi1_1 siRNA were analyzed by Western blotting. These samples correspond to the time points after release from a G1/S block as shown in Fig. 3 A. Actin and Hsp70 are shown as loading controls. The asterisk marks residual Emi1 signal. The same extracts were run on two different gels (top and bottom), and a loading control is shown for each gel. Results are representative of two experiments. (C) HeLa cells were transfected with either control (siCTR) or Emi1 (siEmi1_1) siRNA oligonucleotides, and, 24 h after transfection, MG132 was added for 6 h. Actin is shown as a loading control. The asterisk denotes residual cyclin A signal.