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. 2007 May 7;177(3):425–437. doi: 10.1083/jcb.200611166

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Cyclins A and B1 and geminin levels are reduced, whereas cyclin E increases in rereplicating cells induced by Emi1 knockdown. (A) Asynchronous HeLa cells were transfected with control (siCTR) or Emi1 (siEmi1_1) siRNA duplexes, and samples were collected 24, 48, or 72 h after transfection. Hsp70 is shown as a loading control. The asterisk denotes residual cyclin B1 signal. The same extracts were run on three different gels, and a loading control is shown for each gel. Results are representative of three independent experiments. (B) Asynchronous HeLa cells were transfected with either control (siCTR) or Emi1 (siEmi1_1) siRNA oligonucleotides, and cell ploidy was analyzed by flow cytometry. DMSO or roscovitine was added or not added 24 h after transfection, and cells were analyzed 24 h later. Results are representative of two independent experiments. (C) Cells were transfected as in A to analyze geminin, CDT1, and cdc6 levels. Actin is shown as a loading control. The asterisk denotes residual CDT1 signal. Results are representative of two independent experiments.