Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 May 7;177(3):413–424. doi: 10.1083/jcb.200701122

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — hSgo2 specifies localization of MCAK to the inner centromere. (A) Prometaphase cells after transfection with control and hSgo2 siRNAs were costained for hSgo2, MCAK, and ACA. Chromosomes were stained with DAPI. (B) Comparison of the intensity ratios of MCAK (left) and MCAK normalized to ACA (right) between control and hSgo2-depleted cells (n > 30). (left) The depletion efficiency of MCAK siRNA. (right) Ratios of the intensity of MCAK to ACA at kinetochores (n > 30) in cells treated with nocodazole. (C) Mitotic cells harvested after transfection with control and hSgo2 siRNAs were probed with anti–CENP-F, anti-hSgo2, and anti-MCAK antibodies. The slower migrating MCAK is hyperphosphorylated (p-MCAK). (D) Cells transfected with control, MCAK, and hSgo2 siRNAs were treated with a high dose of nocodazole to depolymerize microtubules, and then costained for ACA, hSgo2, and MCAK.