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. 2007 Jul 30;178(3):425–436. doi: 10.1083/jcb.200701092

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — uPAR is necessary for hypoxia-induced EMT and collagen remodeling. (A) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 50 h in 21% O₂ (N) or 1.0% O₂ (H). The preparations were immunostained for E-cadherin (green channel), phalloidin (red channel), and DAPI. (B) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 18 h on coverslips precoated with fluorescein-labeled type I collagen in 21% O₂ (N) or 1.0% O₂ (H). Cell nuclei are stained with DAPI. Bars, 50 μm.