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. 2007 Jul 30;178(3):425–436. doi: 10.1083/jcb.200701092

Figure 6.

Figure 6.

Rac1 is necessary for hypoxia-induced cell migration, invasion, and collagen remodeling. (A) MDA-MB-468 cells were transfected to express DN-MEK1 and GFP or GFP alone. Cells were allowed to migrate for 24 h in Transwell chambers (cell migration) or in chambers with reconstituted Matrigel (cell invasion). Experiments were performed in 21% O2 (gray bars) or 1.0% O2 (black bars). Migration and invasion are compared with the levels observed with normoxic control cells that expressed GFP (mean ± SEM; n = 3). (B) MDA-MB-468 cells were treated with 100 μM CoCl2 (black bars) or with vehicle (gray bars). The same cells also were treated with 50 μM PD098059 or vehicle (control), as indicated. Transwell migration proceeded for 24 h. Cell migration is expressed relative to that observed with cells treated with vehicle (mean ± SEM; n = 3). (C) MDA-MB-468 cells were transfected to express DN-Rac1 and GFP or GFP alone. Cells were allowed to migrate for 24 h in Transwell chambers (cell migration) or in chambers with reconstituted Matrigel (cell invasion) in 21% O2 (gray bars) or 1.0% O2 (black bars). Results are expressed relative to that observed in normoxic control cells that expressed only GFP (mean ± SEM; n = 6). (D) Cells were transfected to express DN-Rac1 and GFP or GFP alone and cultured for 18 h on collagen labeled with Alexa Fluor 594 in 21% O2 (normoxia) or 1.0% O2 (hypoxia). Nuclei are stained with DAPI. Bar, 50 μm.