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. 2007 Jul 30;178(3):363–369. doi: 10.1083/jcb.200703047

Figure 1.

Figure 1.

A subset of RabGAPs can block primary cilium formation. (A) hTERT-RPE1 cells expressing human GFP-RabGAPs were induced to form primary cilia by serum starvation and then stained for acetylated tubulin (Ac-tubulin) as a marker for primary cilia. Primary cilium formation was counted (n = 100) and is plotted for a representative series of experiments in the bar graph. The blue line marks the mean extent of cilium formation, and the red line is the 40% cutoff used to assign positive GAPs. TBC1D3 (asterisk) caused reduced cell viability and increased levels of apoptosis; TBC1D12, RUTBC1, RUTBC2, USP6, AK074305, and KIAA0882 gave similar effects and are not shown. (B) Images showing the effects of expressing EVI5like, TBC1D7, and XM_037557 on primary cilia formation. Note the lack of a primary cilium and only residual basal body staining (arrows). EVI5 is shown as a negative control where primary cilium formation is normal (arrow). (C) hTERT-RPE1 cells expressing human GFP-tagged TBC1D7, XM_037557, or the inactive XM_037557R140A mutant were induced to form primary cilia by serum starvation and then stained for γ-tubulin as a marker for the basal body or acetylated tubulin as a marker for the cilium (arrows). DNA was stained with DAPI. Bars, 10 μm.