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. 2007 Jul 30;178(3):363–369. doi: 10.1083/jcb.200703047

Figure 3.

Figure 3.

Rab8a is the sole Rab present on primary cilia. (A) hTERT-RPE1 cells expressing human GFP-Rab8a (green) were grown in normal serum or induced to form primary cilia by serum starvation and then stained for acetylated tubulin (red) as a marker for primary cilia. (B) hTERT-RPE1 cells grown in normal serum or induced to form primary cilia by serum starvation were stained for Rab8a (green) and acetylated tubulin (red). (C) The length of primary cilia, defined by acetylated tubulin (Ac-Tub) staining, was measured in control hTERT-RPE1 cells (2.6 ± 0.8 μm) and hTERT-RPE1 cells stably expressing GFP-tagged Rab8a (4.8 ± 0.9 μm). The length of the Rab8a-positive structure (GFP) was also measured (6.7 ± 1.5 μm). These numbers are plotted on the graph with bars to show the standard from the mean (n > 100). (D) hTERT-RPE1 cells expressing Rab8b, -17, and -23 tagged with GFP (green) were induced to form primary cilia by serum starvation and then stained for acetylated tubulin (red) as a marker for primary cilia. DNA was stained with DAPI (blue). Bars, 10 μm.