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. 2007 Jul 30;178(3):363–369. doi: 10.1083/jcb.200703047

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Rab8a is required for primary cilium formation. (A and B) hTERT-RPE1 cells treated with control, IFT20, Rab8a, or GMAP210 siRNA duplexes were induced to form primary cilia by serum starvation for 48 h. The cells were fixed and stained for acetylated tubulin (red) as a marker for primary cilia and IFT20 or Rab8a (green). DNA was stained with DAPI (blue). Bar, 10 μm. (C) The number of cells forming primary cilia was counted in cells treated as indicated in the figure. The results are plotted as a bar graph, and the standard error is shown (n = 3; 100 cells per condition).