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. 2007 Jul 30;178(3):411–423. doi: 10.1083/jcb.200701023

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Loss of vsk3 doss not incur ultrastructural change in vesicles. Vegetative wild-type cells (WT-control), WT cells expressing VSK3ΔK-YFP (VSK3ΔK/WT), and vsk3 ⁻ cells expressing VSK3ΔK-YFP (VSK3ΔK/vsk3 ⁻), which does not rescue the null phenotype, were fixed, probed with a rabbit anti-GFP antibody followed by a gold-conjugated secondary antibody to reveal VSK3-containing vesicles in electron micrographs. Arrows indicates the vesicles. Low and high magnifications are shown.