Table 3.

Reactivity of PBMCs from patients immunized with 209-2M peptide in IFA

		Before immunization				After immunization*
		Assay stimulator†(pg IFN-γ/ml)
Patient	Expt.	T2	T2(280)	T2(209)	T2-(209-2M)	T2	T2(280)	T2(209)	T2(209-2M)
1	1	21	22	12	20	42	37	6,897	57,060
2	1	58	56	66	50	54	48	1,851	4,012
	2	1	4	2	ND‡	30	22	>1,000	ND
3	1	33	26	33	35	46	53	56	54
	2	145	127	124	ND	49	36	46	ND
4	1	133	123	184	213	40	35	2,631	6,086
	2	50	36	41	43	45	52	1,618	3,751
	3	351	299	501	470	86	825	944	1,057
5	1	28	30	21	24	41	35	4,366	6,402
	2	154	156	152	142	128	129	295	323
	3	29	18	37	32	27	22	856	1,126
6	1	38	28	24	31	44	47	152	671
	2	154	166	210	153	128	72	662	887
	3	96	61	117	127	22	14	2,374	5,407
7	1	44	66	72	82	104	81	4,424	5,411
	2	10	8	13	16	197	224	1,293	1,583
	3	127	105	ND	120	61	67	1,244	ND
8	1	ND	ND	ND	ND	17	25	845	ND
	2	1553	562	719	ND	79	78	2,326	ND
	3	ND	ND	ND	ND	43	ND	1,768	ND
9	1	345	337	355	ND	209	183	2,253	ND
	2	13	13	10	ND	229	262	1,550	ND
	3	1434	816	513	ND	495	517	2,408	ND
10	1	247	283	413	ND	29	39	1,271	ND
	2	135	102	146	ND	86	74	24,150	ND
	3	117	147	150	ND	6	9	39,690	ND
11	1	53	53	56	ND	65	71	154	ND
	2	46	50	47	ND	29	39	63	ND
	3	ND	ND	ND	ND	87	83	205	ND

^*Patient 1 after one immunization; all other patients after two immunizations.

^†Day 11 to 13 after culture with 209-2M peptide, PBMCs were tested fro IFN-γ release following 24-h incubation with peptide-pulsed T2 cells.

^‡Not done.