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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Gene. 2007 Mar 27;396(1):134–148. doi: 10.1016/j.gene.2007.03.010

Fig. 5.

Fig. 5

Overexpressed zebrafish caspase-8 induces apoptosis in human HeLa cells.

(A-F) Control plasmid vector pME18S (A and B) or pME18S-Flag/zCasp8 carrying zebrafish casp8 cDNA (C and D) or pME18S-hCASP8 carrying human CASP8 cDNA (E and F) were transfected along with a reporter plasmid pJ7-LacZ into HeLa cells in the absence (A, C and E) or presence (B, D and F) of 100 μM z-VAD-fmk, a peptide caspase inhibitor. After 24 h, cells were fixed, stained with X-Gal and photographed under the microscope. Viable transfectants were observed as βGal-positive blue cells.