Expression of zebrafish caspase-8 in embryos.
The expression profile of caspase-8 during embryonic development. RT-PCR analysis of caspase-8 transcripts was performed with total RNA isolated from embryos collected at 1, 6, 18, 36 and 48 h after fertilization (lanes 1-5). Aliquots of the synthesized first strand cDNAs were amplified with primers for casp8 (Upper) or EF1α as a control (Bottom) and analyzed by agarose-gel electrophoresis. No template DNA and zebrafish casp8 cDNA samples as negative and positive controls were examined in the lanes 6 and 7, respectively.