Table 3.
Prediction of membrane-association of AtUGT85A encoded-proteins and AtGUS encoded-proteins. The presence of N-terminal signal peptide was determined by SignalP 3.0 Server (http://www.cbs.dtu.dk/services/SignalP/). The certainty to be membrane-associated was determined by using WoLF PSORT Prediction (http://psort.nibb.ac.jp/). The PsUGT1-encoded protein is membrane-associated [21]. The AtGUS1-encoded protein has an 85% chance of being extracellularly secreted.
| xKQxxEF (Lysosomal/Golgi Loc. Sig.) | ER membrane retention signal | |
|---|---|---|
| AtUGT85A1 | present | QKSQ at C-terminus |
| AtUGT85A3 | present | GSRF at N-terminus/KIPN at C-terminus |
| AtUGT85A5 | present | absent |
| AtUGT85A2 | present | absent |
| AtUGT85A4 | present | absent |
| AtUGT85A7 | present | absent |
| PsUGT1 | present | absent |
| N-terminal hydrophobic signal peptide | ||
| AtGUS1 | present | |
| AtGUS2 | present | |
| AtGUS3 | present |