Table 4.
Post-translational modification of AtUGT85A and AtGUS-encoded proteins. Prediction of O-β-GlcNAc attachment sites was determined by YinOYang 1.2 program (http://www.cbs.dtu.dk/services/YinOYang/). Prediction of phosphorylation sites was determined by NetPhos 2.0 Server (http://www.cbs.dtu.dk/services/NetPhos/). O-β-GlcNAc = N-acetyl glucosamine.
| Conserved UDP-GA binding site | No. of O-b-GlcNAc attachment sites | No. of Serine phosphorylation | No. of Threonine phosphorylation | No. of Tyrosine phosphorylation | |
|---|---|---|---|---|---|
| AtUGT85A1 | present | 4 | 5 | 5 | 2 |
| AtUGT85A3 | present | 6 | 10 | 6 | 1 |
| AtUGT85A5 | present | 3 | 12 | 5 | 2 |
| AtUGT85A2 | present | 3 | 12 | 5 | 3 |
| AtUGT85A7 | present | 4 | 14 | 4 | 3 |
| AtUGT85A4 | present | 7 | 8 | 6 | 3 |
| PsUGT1 | present | 2 | 4 | 5 | 3 |
| Leucine zipper motif | No. of O-β-GlcNAc attachment sites | No. of Serine phosphorylation | No. of Threonine phosphorylation | No. of Tyrosine phosphorylation | |
| AtGUS1 | present | 5 | 8 | 4 | 8 |
| AtGUS2 | present | 7 | 14 | 4 | 8 |
| AtGUS3 | absent | 5 | 11 | 5 | 6 |