Table 1.
Target complementary regions and unique primer sequences for the PRI-lock probes.
| Targeted species/group | 5' Target complementary sequence (5'-3') | 3' Target complementary sequence (5'-3') | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') |
| Phytophthora spp. | TATCTAGTTAAAAGCAGAGACTTTCGTC | CTGCTGAAAGTTGC | TACGAACGTCTTAGCACTCC | GGTGTTGATTCGCGTCTACT |
| P. infestans | TCGATTCGTGGTATGGTTGGCTTCGGCT | CGTTAATGGAGAAATGC | AGAGTCGGTAGGCACTATGG | CGTATGTCGAATGCAGCTGA |
| R. solani AG 2-2 | TCTGCCTCACAGGTTCACAGGTGTGTGTGG | TTCCCGTCCATGTC | GAGTTCCCGTGCGTTAGATC | TACGGCGCTTGGGACATGAT |
| R. solani AG 4-1 | GGTCCAATAAAGTTCCTTCCCCCCTAGAAAA | AGTCCAA_GGAGAGTA___ | CGTGTCCATCGAGCTGCATA | GACGGCATTCAGAGTACGCT |
| R. solani AG 4-2 | GACTTCTGTCTACTTAATTCATATAAACTCAATT | CTT_CTACTCCCCCTT_ | CTGCCTGTGACTCGTGTATC | AGACCGTATCGTCCACAGTG |
| F. oxysporum | GCGAGTCCCAACACCAAGCTGTGCTTG | GGAACGCGAATTAAC_ | CTGGTGCATGTACTCGACTG | ATCAGATCGACTCGGTAGCT |
| M. roridum | CGGTGGTGGCCATGCCGTAAAACACC | ACTCGCATTGGAGCT | CATCCAGCTCAACGTATCCA | CCTACTGTGACGCTGTGATG |
| V. dahliae | TTTATACCAACGATACTTCTGAGTGTT | CATCAGTCTCTCTG | ATCTGGATCAACGTCGCGCT | ATACAGTCGTCGGGGTCGAA |
| V. alboatrum/V. tricorpus | TTTATACCAACGATACTTCTGAGTGTTCTTAGTGAAC | GTACATCAGTCTCTTTA | GCATCGGGTTCACGCCTATA | TGAAGCACTGACACGCGAAG |
| M. hapla | GTTTATCGTTGTGAATGGCTGTCGCTGGTG | ATTCGAATAGTCTCAAC | TATGGGTCTTGCTGATACGC | TCCGTCTGTTGAGTTAGGCC |
| E. carotovora carotovora | AAAACCTGTGCGTTCATCGATGCTGAACAT | TCAACGCGAAGGA | AGAATCGTACACGCTGCTGG | AATACGACTGACACGAGCTG |
| A. tumefaciens | TCCGGTTGATAGTTGAGGACAGCATTGGAC | GTTGGTCGTCCGCT | CAATACCTGTGACGAGCTGG | ACCCGGTCACTCAGCATATA |
| G. Proteo bacterial spp. | GGCCTTCTTCACACACGCGGCATGGCTGCA | GCTTTACAACCCGAA | ACAGGTCATCGAACTCTCAC | AGAACACGTCAGAGGTCCGT |
| Internal Ligation Control (ILC) | GGGAGAACACTGCGTGGTTTTCACATAC | GCTTGTGCCTCTCGA | CTATCGCGTGCTAGTCGTCT | ATTCTAATCAATCGTCGCGG |
Nucleotides highlighted in bold font, indicate polymorphism within the target group. Nucleotides or gaps owing to deletions used to discriminate from most similar, non-target sequences, are underlined. (No such sequence was found for the PRI-lock probes G. Proteo bacterial spp. and A. tumefaciens)