Table 4.

Quantification and sensitivity of the developed assay on the Biotrove OpenArray™ platform.

PRI-lock probe	Calibration curve formula	R2 value	Detection limit (copy number/μl ligation mixture)	Linear quantification range (copy number/μl ligation mixture)
Phytophthora spp.	y = -3.61x + 38.1	0.995	104	108 – 104
P. infestans	y = -3.31x + 35.7	0.989	103	108 – 103
R. solani AG 2-2	y = -3.49x + 37.9	0.994	104	108 – 104
R. solani AG 4-1	y = -3.49x + 35.8	0.994	103	109 – 103
R. solani AG 4-2	y = -3.35x + 39.2	0.998	103	108 – 104
F. oxysporum	y = -3.47x + 39.1	0.990	104	108 – 104
M. roridum	y = -3.15x + 36.6	0.984	104	109 – 104
V. dahliae	y = -3.42x + 36.3	0.996	103	108 – 103
V. alboatrum/V. tricorpus	y = -3.40x + 38.8	0.991	103	108 – 104
M. hapla	y = -3.39x + 38.1	0.989	104	108 – 104
E. carotovora carotovora	y = -3.51x + 36.3	0.997	103	109 – 103
A. tumefaciens	y = -3.36x + 36.5	0.995	103	108 – 103
G. Proteo bacterial spp	y = -3.51x + 38.1	0.995	104	108 – 104

(x) = Log₁₀(copy number target input/μl ligation mixture). (y) = C_T value.