Table 5.
PRI-lock assay validation. Analysis of target DNAs mixed in different ratios.
| Template (Log10 target (copy number/μl ligation reaction)) |
Ratio | Observed CT values (SD) in PRI-lock specific PCR | Observed pathogen template (SD) (Log10 target (copy number/μl ligation reaction)) |
|||
| P. sojae | E. carotovora carotovora | Phytophthora spp. | E. carotovora carotovora | Phytophthora spp. | E. carotovora carotovora | |
| 8 | 5 | 1000: 1 | 9.4 (0.30) | 19.3 (0.23) | 7.9 (0.08) | 4.9 (0.07) |
| 8 | 4 | 10000: 1 | 9.3 (0.06) | 22.7 (0.34) | 8.0 (0.02) | 3.9 (0.10) |
| 5 | 8 | 1: 1000 | 20.6 (0.20) | 8.8 (0.14) | 4.8 (0.06) | 7.9 (0.04) |
| 4 | 8 | 1: 10000 | 24.2 (0.80) | 8.9 (0.07) | 3.8 (0.22) | 7.8 (0.02) |
Target numbers were calculated using the CT values and the corresponding calibration curves. Data represent 2 ligation replicates (n = 2), each with 4 qPCR replicates (n = 4).