Fig. 2.

Bulk fluorescence emission and absorption spectra revealed proper reconstitution of mononucleosomes. a Bulk fluorescence emission spectra. A distinct peak of fluorescence at the acceptor emission wavelength was seen after reconstitution, which was not present for the labeled DNA only. The peak disappeared upon dilution of the reconstituted material in 2M NaCl, an ionic strength at which nucleosome structure is disrupted. b Bulk absorption spectrum of the fluorescently labeled DNA construct. The stoichiometry of the labels was obtained by comparison with the absorption spectra of ATTO550 and ATTO647N (as provided by the manufacturer), which are plotted with dotted lines