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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 12;6(10):1507–1516. doi: 10.1016/j.dnarep.2007.04.014

Figure 3. Nej1-Lif1 and XLF-XRCC4 minimal interacting regions, two-hybrid analysis.

Figure 3

Wild-type haploid yeasts containing various bait and prey constructs were mated, spotted to two-hybrid indicator medium lacking adenine or histidine, and grown for 5 days at 30° C. Prey columns labeled “vector” contained no insert to show the level of bait auto-activation. Panels show the most important bait and prey combinations from the overall analysis. Results are summarized in Figure 2. Panels show heterotypic interactions between (A) Nej1 and Lif1 and (B) and (C) XLF and XRCC4, spotted to adenine-selective medium, as well as homotypic interactions of (D) Nej1 and (E) XRCC4, spotted to the more permissive histidine-selective medium.