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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 12;6(10):1507–1516. doi: 10.1016/j.dnarep.2007.04.014

Figure 5. Differential stability of homotypic and heterotypic XLF/Nej1 and XRCC4/Lif1 interactions.

Figure 5

XLF/Nej1 and XRCC4/Lif1 were co-expressed in wild-type diploid yeast as combinations of CBP- and FLAG-tagged proteins. Note that the two tags were on separate expression constructs even when the same protein was tagged twice. CBP-tagged proteins and any interacting proteins were pulled-down from cell-lysates with calmodulin agarose at 0.15 M (left panels) or 0.5 M (middle panels) NaCl and immunoblotted as in Figure 4. For relevant lanes, band intensities were determined using the LiCor Odyssey scanner, corrected for any background of non-specifically bound protein, and expressed as a ratio of FLAG to CBP signals. For both (A) yeast proteins and (B) human proteins, the XLF/Nej1-XRCC4/Lif1 heterotypic interaction was substantially more sensitive to high salt than the strong and stable Nej1 and XRCC4 homotypic interactions.