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. 1992 Sep;174(17):5727–5731. doi: 10.1128/jb.174.17.5727-5731.1992

Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdin.

P Visca 1, L Serino 1, N Orsi 1
PMCID: PMC206521  PMID: 1512205

Abstract

We have isolated and characterized by chemical and enzymatic analyses three distinct types of pyoverdin-defective (pvd) mutants of Pseudomonas aeruginosa PAO1. The pvd-1 mutant is an L-N5-hydroxyornithine (L-N5-OH-Orn) auxotroph unable to hydroxylate L-ornithine (L-Orn) in a cell-free system and requiring L-N5-OH-Orn for pyoverdin production. The other two types of mutants appear to be blocked in further steps of the biosynthetic pathway leading to pyoverdin, namely, the acylation of L-N5-OH-Orn (pvd-2) and chromophore synthesis (pvd-3). The different pvd mutations were all found to be located in the catA1 region at 47 min of the genetic map of P. aeruginosa PAO1.

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Selected References

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  1. Akers H. A., Llinás M., Neilands J. B. Protonated amino acid precursor studies on rhodotorulic acid biosynthesis in deuterium oxide media. Biochemistry. 1972 Jun 6;11(12):2283–2291. doi: 10.1021/bi00762a012. [DOI] [PubMed] [Google Scholar]
  2. Ankenbauer R., Hanne L. F., Cox C. D. Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production. J Bacteriol. 1986 Jul;167(1):7–11. doi: 10.1128/jb.167.1.7-11.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ankenbauer R., Sriyosachati S., Cox C. D. Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin. Infect Immun. 1985 Jul;49(1):132–140. doi: 10.1128/iai.49.1.132-140.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Atkin C. L., Neilands J. B. Rhodotorulic acid, a diketopiperazine dihydroxamic acid with growth-factor activity. I. Isolation and characterization. Biochemistry. 1968 Oct;7(10):3734–3739. doi: 10.1021/bi00850a054. [DOI] [PubMed] [Google Scholar]
  5. Callahan L. T., 3rd Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum. Infect Immun. 1976 Jul;14(1):55–61. doi: 10.1128/iai.14.1.55-61.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colonna B., Nicoletti M., Visca P., Casalino M., Valenti P., Maimone F. Composite IS1 elements encoding hydroxamate-mediated iron uptake in FIme plasmids from epidemic Salmonella spp. J Bacteriol. 1985 Apr;162(1):307–316. doi: 10.1128/jb.162.1.307-316.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cornelis P., Hohnadel D., Meyer J. M. Evidence for different pyoverdine-mediated iron uptake systems among Pseudomonas aeruginosa strains. Infect Immun. 1989 Nov;57(11):3491–3497. doi: 10.1128/iai.57.11.3491-3497.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cox C. D., Graham R. Isolation of an iron-binding compound from Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):357–364. doi: 10.1128/jb.137.1.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cox C. D., Rinehart K. L., Jr, Moore M. L., Cook J. C., Jr Pyochelin: novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4256–4260. doi: 10.1073/pnas.78.7.4256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Emery T. F. Initial steps in the biosynthesis of ferrichrome. Incorporation of delta-N-hydroxyornithine and delta-N-acetyl-delta-N-hydroxyornithine. Biochemistry. 1966 Nov;5(11):3694–3701. doi: 10.1021/bi00875a045. [DOI] [PubMed] [Google Scholar]
  11. Gross R., Engelbrecht F., Braun V. Genetic and biochemical characterization of the aerobactin synthesis operon on pColV. Mol Gen Genet. 1984;196(1):74–80. doi: 10.1007/BF00334095. [DOI] [PubMed] [Google Scholar]
  12. Guterman S. K. Colicin B: mode of action and inhibition by enterochelin. J Bacteriol. 1973 Jun;114(3):1217–1224. doi: 10.1128/jb.114.3.1217-1224.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  14. Heinrichs D. E., Young L., Poole K. Pyochelin-mediated iron transport in Pseudomonas aeruginosa: involvement of a high-molecular-mass outer membrane protein. Infect Immun. 1991 Oct;59(10):3680–3684. doi: 10.1128/iai.59.10.3680-3684.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Herrero M., de Lorenzo V., Neilands J. B. Nucleotide sequence of the iucD gene of the pColV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions. J Bacteriol. 1988 Jan;170(1):56–64. doi: 10.1128/jb.170.1.56-64.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Isaac J. H., Holloway B. W. Control of arginine biosynthesis in Pseudomonas aeruginosa. J Gen Microbiol. 1972 Dec;73(3):427–438. doi: 10.1099/00221287-73-3-427. [DOI] [PubMed] [Google Scholar]
  17. LIN E. C., LERNER S. A., JORGENSEN S. E. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochim Biophys Acta. 1962 Jul 2;60:422–424. doi: 10.1016/0006-3002(62)90423-7. [DOI] [PubMed] [Google Scholar]
  18. Marugg J. D., van Spanje M., Hoekstra W. P., Schippers B., Weisbeek P. J. Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358. J Bacteriol. 1985 Nov;164(2):563–570. doi: 10.1128/jb.164.2.563-570.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matsumoto H., Nakazawa T., Ohta S., Terawaki Y. Chromosomal locations of catA, pobA, dcu and chu genes in Pseudomonas aeruginosa. Genet Res. 1981 Dec;38(3):251–266. doi: 10.1017/s0016672300020590. [DOI] [PubMed] [Google Scholar]
  20. Meyer J. M., Hohnadel D., Khan A., Cornelis P. Pyoverdin-facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdin receptor. Mol Microbiol. 1990 Aug;4(8):1401–1405. doi: 10.1111/j.1365-2958.1990.tb00719.x. [DOI] [PubMed] [Google Scholar]
  21. Moore A. T., Nayudu M., Holloway B. W. Genetic mapping in Methylophilus methylotrophus AS1. J Gen Microbiol. 1983 Mar;129(3):785–799. doi: 10.1099/00221287-129-3-785. [DOI] [PubMed] [Google Scholar]
  22. Moores J. C., Magazin M., Ditta G. S., Leong J. Cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting Pseudomonas strain. J Bacteriol. 1984 Jan;157(1):53–58. doi: 10.1128/jb.157.1.53-58.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Morgan A. F. Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79. J Bacteriol. 1979 Jul;139(1):137–140. doi: 10.1128/jb.139.1.137-140.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Murray G. J., Clark G. E., Parniak M. A., Viswanatha T. Effect of metabolites on epsilon-N-hydroxylysine formation in cell-free extracts of Aerobacter aerogenes 62-1. Can J Biochem. 1977 Jun;55(6):625–629. doi: 10.1139/o77-090. [DOI] [PubMed] [Google Scholar]
  25. Poole K., Neshat S., Heinrichs D. Pyoverdine-mediated iron transport in Pseudomonas aeruginosa: involvement of a high-molecular-mass outer membrane protein. FEMS Microbiol Lett. 1991 Feb;62(1):1–5. [PubMed] [Google Scholar]
  26. Rombel I. T., Lamont I. L. DNA homology between siderophore genes from fluorescent pseudomonads. J Gen Microbiol. 1992 Jan;138(1):181–187. doi: 10.1099/00221287-138-1-181. [DOI] [PubMed] [Google Scholar]
  27. Royle P. L., Matsumoto H., Holloway B. W. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1981 Jan;145(1):145–155. doi: 10.1128/jb.145.1.145-155.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schwyn B., Neilands J. B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987 Jan;160(1):47–56. doi: 10.1016/0003-2697(87)90612-9. [DOI] [PubMed] [Google Scholar]
  29. Sokol P. A., Woods D. E. Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa. Infect Immun. 1983 May;40(2):665–669. doi: 10.1128/iai.40.2.665-669.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang J., Budde A. D., Leong S. A. Analysis of ferrichrome biosynthesis in the phytopathogenic fungus Ustilago maydis: cloning of an ornithine-N5-oxygenase gene. J Bacteriol. 1989 May;171(5):2811–2818. doi: 10.1128/jb.171.5.2811-2818.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Watson J. M., Holloway B. W. Suppressor mutations in Pseudomonas aeruginosa. J Bacteriol. 1976 Mar;125(3):780–786. doi: 10.1128/jb.125.3.780-786.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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