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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: J Immunol Methods. 2007 Aug 8;326(1-2):93–115. doi: 10.1016/j.jim.2007.07.012

Fig 7.

Fig 7

Decreased naïve cells after long-term cryopreservation. (a) Naïve cells are defined as CD45RA+ CD28+; central memory cells as CD45RA− CD28+ and effector memory cells as CD45RA+/− CD28−. Summary results are gated on viable (b) CD3+CD4+ and (d) CD3+CD4−" unstimulated PBMC from fresh and long-term cryopreserved (>300 days) samples from the same phlebotomy. Two-tailed, paired t tests were performed with significant p values highlighted in bold. Bland−Altman plots showing the difference of fresh PBMC (naïve, central memory or effector memory) minus long-term cryopreserved PBMC (naïve, central memory or effector memory) vs. the average are shown for (c) CD4+ and (e) CD8+ T cell phenotypes. The bias is plotted with a solid line and 95% limits of agreement with dotted lines.