Table 1. Details of the experimental conditions tested and summary of the antitumor effects achieved.
Exp | N | t [µs] | U/d [V/cm] | d [mm] | f [Hz] | Tmax [°C] | t43 [s] | CR |
α: IMMUNOCOMPETENT MICE (Figure 1) | ||||||||
1B | 1 | 800 | 2000 | 3 or 5 | 1 | 39 | 0.01 | 0/7 (0%) |
1C | 8 | 100 | 2000 | 3 or 5 | 1 | 38 | 0.002 | 0/7 (0%) |
1E | 8 (4×2) | 800 | 2000 | 5 | 1 | 43 | 0.6 | 0/6 (0%) |
1F | 64 (16×4) | 100 | 2000 | 5 | 5000 | 40 | 0.03 | 1/6 (17%) |
1H | 8 (4×2) | 800 | 2500 | 4 | 1 | 46 | 30 | 3/6 (50%) |
1I | 64( 16×4) | 100 | 2500 | 4 | 5000 | 41 | 0.3 | 2/6 (33%) |
1K | 64(16×4) | 100 | 2500 | 4 | 1 | 38 | 0.004 | 3/8 (37%) |
1L | 8 (4×2) | 1000 | 2500 | 4 | 0.3 | 51 | 1300 | 5/8 (62%) |
β: IMMUNODEFICIENT MICE (Figure 2) | ||||||||
2B | 8 (4×2) | 1000 | 2500 | 4 | 0.3 | 51 | 1300 | 2/13 (15%) |
2C | 80 (4×20) | 100 | 2500 | 4 | 3 | 40 | 0.1 | 11/16 (69%) |
2E | 8 (4×2) | 1000 | 2500 | 4 | 0.03 | 40 | 0.05 | 4/13 (31%) |
2F | 80 (4×20) | 100 | 2500 | 4 | 0.3 | 37.5 | 0.001 | 12/13 (92%) |
In all experiments except 1B and 1C, the pulses were delivered in 2 perpendicular orientations. The EP were delivered using a Cliniporator in all conditions except 1L, 2B and 2F for which a Jouan device was used (see Methods). Exp: experimental condition; n: number of pulses; t: individual pulse duration; U/d: voltage to distance ratio (approximate electric field strength); d: distance between the plate electrodes; f: repetition frequency; Tmax: theoretical maximum temperature; t43: theoretical equivalent thermal dose; CR: number of complete regressions/number of treated tumors, and percentage of CR.