Ovaries from PND4 B6C3F1, CYP 2E1 +/+ or -/- mice were cultured with medium control or 1,2-VCM (125, 250, 500, 750, or 1000 μM) for 15d. Following incubation, ovaries were collected, and processed for histological evaluation as described in materials and methods. Healthy (A) primordial, and (B) primary follicles were classified and counted. Values are mean ± SE total follicles counted/ovary, n=5; different letters differ (p<0.05) from one another within each follicle type.