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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Protein Expr Purif. 2007 Jun 20;56(1):62–71. doi: 10.1016/j.pep.2007.06.002

Table 1.

Functionality and yield of purified Ste2p-rho from 1 L culture.

Receptor and Strain Growth arrest assay (%) Kd (nM) Bmax or Yield
WT (C59S, C252S) in LM102 100 ± 12 8.9 ± 0.6 75,300 ± 6800 per cell
Ste2p-rho in LM102 104 ± 7 12.7 ± 1.2 36,800 ± 3900 per cell
Purified Ste2p-rho from BJS21 Not Applicable 27.8 ± 3 120 μg/100 mg membrane (26 pmole/mg)
39 ± 7 % active receptora
a

Active population of the purified receptor was calculated from the Bmax value in saturation binding assays. Specific activity of [3H]α-factor was 16 Ci/mmol and the average Bmax value of four separate binding assays was 6,000 cpm/40 ng receptor.