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. Author manuscript; available in PMC: 2007 Nov 7.

Published in final edited form as: Insect Biochem Mol Biol. 2007 Aug 7;37(11):1222–1233. doi: 10.1016/j.ibmb.2007.07.015

Developmental- and tissue-specific expression of each IAP1 mRNA variant from Cx. pipiens. (A) RT-PCR amplifications were performed using mRNA variant-specific primers and total RNA extracted from various life stages, namely: 24-h-old embryos (lane 1), first/second instar larvae (lane 2), third instar larvae (lane 3), fourth instar larvae (lane 4), early pupae (lane 5), late pupae (lane 6) and adults (lane 7). (B) RT-PCR amplifications were performed using mRNA variant-specific primers and total RNA extracted from various adult female tissues, namely midguts (lane 1) ovaries (lane 2) and salivary glands (lane 3). Control reactions were performed using primers specific to the Cx. pipiens actin cDNA sequence

Developmental- and tissue-specific expression of each IAP1 mRNA variant from Cx. pipiens. (A) RT-PCR amplifications were performed using mRNA variant-specific primers and total RNA extracted from various life stages, namely: 24-h-old embryos (lane 1), first/second instar larvae (lane 2), third instar larvae (lane 3), fourth instar larvae (lane 4), early pupae (lane 5), late pupae (lane 6) and adults (lane 7). (B) RT-PCR amplifications were performed using mRNA variant-specific primers and total RNA extracted from various adult female tissues, namely midguts (lane 1) ovaries (lane 2) and salivary glands (lane 3). Control reactions were performed using primers specific to the Cx. pipiens actin cDNA sequence