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. 2007 Nov 9;3(11):e200. doi: 10.1371/journal.pgen.0030200

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© 2007 Archambault et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Females heterozygous for both the Scant mutation and a loss-of-function polo mutation (such as polo¹¹) lay embryos that die during development.

(B) Genetic screen to generate Scant revertant (Sr) alleles. Males homozygous for Scant were x-rayed and crossed to polo¹¹ heterozygous females. Female progeny were tested for fertility. Scant revertant mutations restore female fertility and can be duplications of the polo⁺ gene, third-site suppressor mutations (su), or recessive mutations allelic to each other. See Text S1 for details.

(C) Hopping a P-element inserted directly upstream of the gwl gene generated imprecise excisions disrupting either gwl (gwl², gwl^3a, and gwl^6a), CG7718 (CG7718¹, CG7718^6b, and CG7718⁷) or both genes (gwl^3b). gwl², gwl^3a, and gwl^6a are semilethal and produce a “Scant-revertant (Sr) phenotype” when placed over Sr alleles (generated in [B]). The extent of the imprecise excisions was approximately mapped by the ability to PCR-amplify the 1 kb-long regions of genomic DNA defined by the tick marks.