Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Comparison of BIE (in black) and V/K KIE (in blue) for human thymidine phosphorylase. The KIE reported here are for arsenolysis of thymidine. Use of arsenate as the nucleophile introduces an irreversible step following the transition state formation. Binding isotope effects were measured in the presence of sulfate as a non-reactive phosphate mimic [28].
