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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Cell Calcium. 2007 Mar 21;42(2):193–203. doi: 10.1016/j.ceca.2007.02.007

Figure 6.

Figure 6

C. elegans CRAC channel activity is mediated by STIM-1 and ORAI-1. (A) Current-to-voltage relationships of currents observed in a HEK293 with replete stores coexpressing ORAI-1 and STIM-1 and in an ORAI-1/STIM-1 coexpressing HEK293 cell in which stores were depleted. Current-to-voltage relationships were plotted for currents measured 5 min after obtaining whole cell access. Store depletion was induced by dialyzing cells with a pipette solution containing 10 mM BAPTA, 10 μM IP3 and ∼18 nM free-Ca2+. Depletion of stores was prevented using a pipette solution containing 10 mM BAPTA, 2 mM ATP and ∼140 nM free-Ca2+. (B) Effect of stim-1 or orai-1 RNAi on endogenous CRAC channel activity measured in cultured C. elegans intestinal cells. Values are means ± S.E. (n=7-21)