Figure 4. Evaluation of cell migration, invasion and proliferation with respect to TEM7 expression.
Top: Cell migration studies - The low-grade MG63 cells (express very little TEM7) were used for comparison of cell-migration, these cells did not receive any siRNA. Cells were grown in presence of 0% (light bars) and 10% FBS (dark bars). A – migration of SaOS cells; B – migration of TE85 cells. Both SaOS and TE85 cells show drastic inhibition of migration in presence of TEM7 siRNA. In these experiments, both siRNAs were used together. Middle: Invasion studies : A- invasion by SaOS cells; B – invasion by TE85 cells. Invasion by SaOS cells are more affected under TEM7 silencing than in TE85 cells. Bottom: Cell proliferation studies - Cells were grown in presence of 10% FBS. Both siRNAs, together, were used. A ~30% reduction in proliferation is observed for SaOS and TE85 cells, however, the siRNAs had no effect on the proliferation of MG63 cells.