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. 2007 Nov 8;117(12):3879–3889. doi: 10.1172/JCI32614

Figure 4. HIF target gene expression is increased in VhlR/R mice.

Figure 4

(A and B) Serum levels of VEGF and Epo were determined by ELISA; pooled results from mice of varying ages are shown. (A) VEGF levels were 1.3-fold greater in VhlR/R mice than in WT and VhlR/+ mice. *P < 0.002. (B) Epo levels were 1.8-fold greater in VhlR/R mice (P < 0.08). (C–F) TaqMan real-time PCR analysis of HIF gene expression in kidney (C and D), liver (E), and spleen (F). The expression of several genes, most notably HIF-2α–preferential targets such as Glut-1, PAI-1, and VEGF, was increased in tissues isolated from VhlR/R mice compared with those of WT mice. *P < 0.05; **P < 0.007; #P < 0.059 (trending toward significance). Renal Epo mRNA expression was increased 1.3-fold (P < 0.09) in VhlR/R mice with increased serum Epo levels (D).