Figure 8. Erythropoiesis is enhanced in Vhl^R/R spleens in vitro.

(A–C) Erythroid differentiation of Vhl^R/R bone marrow cells was not significantly different from WT (A). Similarly, there was only a small (but not statistically significant) increase in the number of nonerythroid colonies formed by Vhl^R/R bone marrow, most notably CFU-G (B) and CFU-M (C). (D–F) In contrast, Vhl^R/R splenic cells had 3.5-fold higher potential to generate erythroid colonies (D), as well as increased potential to form nonerythroid colonies such as CFU-G and CFU-GEMM (E) and CFU-M (F). *P < 0.02; **P < 0.003; ^#P < 0.059 (trending toward significance). (G and H) Epo dose-response curves of WT and Vhl^R/R cells showing the number of BFU-E colonies formed. Both bone marrow (G) and splenic (H) cells were hypersensitive to Epo.