Figure 6. Targeted knockdown of zebrafish HMG-CoA reductase has a muscle phenotype similar to that with lovastatin treatment and can be rescued by zebrafish atrogin-1 knockdown.

(A) Myosin heavy chain staining of representative control, HMG-CoA reductase, and combined HMG-CoA reductase/atrogin-1 knockdown in zebrafish embryos. Original magnification, ×200. (B) Quantitation of muscle damage. Classes of morphological phenotypes are as described in Figure 5 legend. MS1 and MS2 are missense controls for morpholinos MO1 and MO2, respectively. Note that in each case, the injection of morpholinos against atrogin-1 almost completely abolishes the damage caused by HMG-CoA reductase knockdown. Number of embryos quantitated in each bar are 231, 182, 197, and 202 for control, and 220, 204, 240, and 215 for the atrogin-1 knockdown.