(A) Asci from a ctp1Δ × ctp1Δ mating are abnormal. DNA is stained with DAPI.
(B) Spore viability in a ctp1Δ × ctp1Δ mating is very low. This defect is partially suppressed in a rec12Δ background that is unable to form programmed meiotic DSBs.
(C) Ctp1 is required for HR but not NHEJ. HR was measured by integration of a transformed linearized plasmid into homologous sequences. NHEJ was measured by circularization of a transformed linearized plasmid. Values are calculated with respect to transformants obtained with an uncut plasmid and are normalized to wild type.