Table 3.

Detection of heterozygosity of microsatellite markers JS2 and PLC by PCR, from either single or 3 pooled MDA reactions on 5 FTA discs of HAT cases from the DRC. Heterozygosity was analysed both by visualisation of PCR product on ethidium bromide stained agarose gel, and by separation of FAM-labelled products on an ABI300 sequencer. Individual alleles were identified by size in bp by GENESCAN© software, and allocated an identifying number. The numbers in the table indicate the alleles identified in each case. Homozygous samples are highlighted in bold. CTC count indicates the estimation of trypanosome numbers (trypanosomes per mL) by the CTC technique.

Sample		Markers
		JS2			PLC
		Single	Pooled		Single	Pooled		CTC count
MAL100		1 2	1 2		2 2	1 2		251
MAL200		1 2	1 2		1 2	1 2		251
MAL300		1 2	1 2		1 2	1 2		251
MAL400		1 2	1 2		1 2	1 2		1000
MAL500		1 2	1 2		1 2	1 2		251
MAL600		1 2	1 2		1 2	1 2		1000
MAL800		1 2	1 2		1 2	1 2		251
MAL900		1 2	1 2		2 2	1 2		251
MAL10a		2 2	1 2		2 2	1 2		251
MAL111		1 4	1 4		1 2	1 2		251
MAL122		1 2	1 2		1 1	1 1		501