Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Source |
|---|---|---|
| YEp13 | High-copy (2 μm), LEU2 | Rose and Broach (1990) |
| YEp24 | High-copy (2 μm), URA3 | Rose and Broach (1990) |
| YEplac181 | High-copy (2 μm), LEU2 | Gietz and Sugino (1988) |
| pRS316 | Low-copy (CEN), URA3 | Sikorski and Hieter (1989) |
| pRS315 | Low-copy (CEN), LEU2 | Sikorski and Hieter (1989) |
| pRS426 | High-copy (2 μm), URA3 | Christianson et al. (1992) |
| pPB547 | BEM3 in YEp13 | Bi and Pringle (1996) |
| YEp13-RGA1 | RGA1 in YEp13 | Bi and Pringle (1996) |
| YEp13-ZDS1* | ZDS1 in YEp13 | Bi and Pringle (1996) |
| YEp24-RGA1 | RGAI in YEp24 | Stevenson et al. (1995) |
| pDLB1981 | RGA2 in pRS426 | Gladfelter et al. (2002) |
| YEp181-RGA1(WT) | RGA1 in YEplac181 | See text |
| YEp181-RGA1 (K872A) | rgalK872A in YEplac181 | See text |
| pRS316-CDC3-GFP | CDC3-GFP in pRS316 | See text |
| pRS316-N-MYO1-GFP | MYO1-GFP in pRS316 | See text |
| YEp13-GFP-BEM3 | GFP-BEM3 in YEp13 | See text |
| pRS315-GFP-RGA1 | GFP-RGA1 in pRS315 | See text |