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. 2003 Oct;14(10):4207–4220. doi: 10.1091/mbc.E03-03-0147

Figure 2.

Figure 2.

TI-VAMP forms SNARE complexes with plasma membrane and endosomal t-SNAREs and colocalizes with Stx 7. (A) SNARE complex formation of TI-VAMP in rat brain. The Triton X-100–soluble fraction of rat brain was incubated with mAb 158.2 or control IgGs (Ctr) covalently coupled to protein G-Sepharose. An aliquot of the starting material (SM) and material bound to immunobeads was analyzed by Western blotting with antibodies to TI-VAMP, Vti1b, Stx7, Stx 13, Stx1, and SNAP25. (B) Localization of TI-VAMP and syntaxin 7 in cortical/striatal neurons in culture. E16 rat cortical-striatal neurons were grown for 1 d in vitro, labeled for TI-VAMP and syntaxin 7, and analyzed by confocal microscopy. Vesicular structures positive for TI-VAMP and Stx 7 are indicated by arrows (bar, 5 μm).