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. 2003 Oct;14(10):4207–4220. doi: 10.1091/mbc.E03-03-0147

Figure 6.

Figure 6.

Endocytosed L1 reaches the TI-VAMP compartment but is absent from the Syb 2-compartment. (A) L1 is endocytosed in NGF-differentiated PC12-cells. PC12 cells were grown in the presence of NGF for 48 h. Cells were incubated with L1-specific Fab fragments or control IgGs. The cells were “acid stripped” and processed for immunofluorescence analysis (bar, 8 μm). (B) L1 is taken up into the TI-VAMP compartment but not into the Syb 2 compartment. NGF-differentiated PC12 cells were incubated with L1-specific Fab fragments (green) as described above and labeled for TI-VAMP (red) or Syb 2 (red). Arrows highlight intracellular structures positive for TI-VAMP and endocytosed L1-specific Fab fragments (green) (bar, 5 μm). (C) L1 is taken up into the TI-VAMP compartment in neurons in culture. Cortical-striatal neurons grown on collagen were incubated with Fab-fragments directed against L1. After removal of the Fab fragments, the neurons were further incubated at 37°C, fixed, and labeled for TI-VAMP (red) and endocytosed L1 (green). Arrows point at structures in the growing axon positive for TI-VAMP and endocytosed anti-L1 Fab fragments (bar, 3 μm).