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. 2003 Oct;14(10):4207–4220. doi: 10.1091/mbc.E03-03-0147

Figure 9.

Figure 9.

L1-dependent adhesive contacts induce clustering of TI-VAMP but not Syb 2-positive vesicles in neuronal growth cones. (A) Accumulation of TI-VAMP at bead/growth cone junctions. Hippocampal neurons grown for 3 d in vitro were incubated with L1-coated beads and processed for confocal microscopy analysis with mAb 158.2 (red) and polyclonal antibody to L1 (green). A basal and an apical section of the same region are shown. Note the formation of bead shaped, TI-VAMP–positive structures in the growth cones. (B) Comparison between TI-VAMP and Syb 2 compartment in a growth cone contacting a bead. Hippocampal neurons were incubated with L1 beads and processed for immunofluorescence with mAb 158.2 and polyclonal antibody to Syb 2. A basal and an apical section of the same region are shown and bead positions are indicated by asterisks. Note the strong TI-VAMP immunoreactivity in the apical confocal section as compared with the basal section (bar, 4 μm).