Figure 3.

Localization of nuclear lamins and emerin in gp210-siRNA cells. (A) Immunofluorescence of GL2-siRNA and gp210-siRNA cells double-stained for gp210 plus either lamin B (a), lamin A/C (b), or emerin (c). Images were obtained by laser confocal microscopy. (B) Assay for in vitro import of rhodamine-labeled NLS-BSA into nuclei of digitonin-permeabilized gp210-siRNA cells (left), GL2-siRNA cells (middle), or unpermeabilized cells (right). Each arrow indicates one nucleus. Bars: 20 μm in A and 10 μm in B.