Figure 4.

Gp210 in C. elegans. (A) A schematic diagram of full-length Ce-Gp210 (residues 1–1847), drawn approximately to scale, showing the exons (numbered boxes), a single predicted transmembrane domain (TM), and the bacterial Ig-like type 2 (Big2) domain. Regions used as antigen are marked by orange lines and labeled by serum number (3586 and 3783). (B) Western blot of crude protein extract of mixed stage C. elegans probed with preimmune (P) or immune (I) serum 3586. (C) Confocal images of an adult C. elegans gonad double-stained by indirect immunofluorescence using Ce-Gp210 serum 3783 (left panel), FG-repeat nucleoporins (mAb414; NUPs; middle panel), and the merged images (right panel).