Figure 5.

RNAi-induced downregulation phenotype for Ce-Gp210. (A) Double immunostaining of control embryos (left) or Cegp210(RNAi) embryos (right; exons 8–15 were targeted), imaged by fluorescence microscopy. Embryos were stained by indirect immunofluorescence using antibodies against Ce-Gp210 (serum 3783), Ce-lamin (Liu et al., 2000), or FG-repeat nucleoporins (Nups; mAb414). Arrows indicate NPC clusters. (B) Thin-section TEMs of control untreated embryos (a) and Ce-gp210(RNAi) embryos in which exons 3–5 were targeted (b and c). Cegp210(RNAi) embryos had more condensed chromatin (b), an enlarged nuclear envelope lumen (b), and clustered NPCs (c). NU, nucleus; Cyt, cytoplasm. Bars: 10 μm (A), 200 nm (B, c), 750 nm (B, b), and 1500 nm (B, a).