Table 1.

Differential regulation of Cdc6-wt and p49-tCdc6 cytoplasmic translocation by phosphorylation

	Cdc6-wt			p49-tCdc6
Subcellular location(%)	Mock	CyclinA	Cdk-dn	Mock	CyclinA	Cdk-dn
Nucleus	70.5	5.7	99.4	89.4	61.5	99.9
Cytoplasm	29.5	94.3	0.6	10.6	38.5	0.1
Factors contributing to the cytoplasmic translocation of Cdc6 are as follows:
1) NES: Mock: 29.5% - 10.6% = 18.9%; Cyclin A: 94.3% - 38.5% = 55.8%; Cdk2-dn: No Effect; and
2) Phosphorylation by cyclin A-Cdk2: Cdc6-wt: 99.4% - 5.7% = 93.7%; p49-tCdc6: 99.9% - 61.5% = 38.4%.