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Xenopus sperm centrosomes recruit Ran from Xenopus egg extract. Decoration of Xenopus sperm centrosomes before (A) or after (B) activation in Xenopus egg extracts by using anti-Ran and anti-γ-tubulin antibodies. Before activation (A), Ran was not observed at the centrosome, whereas γ-tubulin was present. After activation, Ran was recruited at centrosomes and codistributed with γ-tubulin (B). Bars, 10 μm.
